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Composition of αβ TCR+ T cell subpopulations in PBMC of naïve chickens. ( A ) Flow cytometry gating scheme for analysis of αβ TCR+ T cells. ( B ) Kinetics of αβ TCR+ T cell subtypes in PBMC of naïve adult chickens from the 6th until the 60th week of age. Upper left: cells expressing CD8α among all αβ TCR expressing cells, upper right: proportion of CD8α+ cells expressing CD8β, lower left: cells expressing CD4 among all αβ TCR expressing cells and lower right: proportion of CD4+ cells expressing <t>CD25.</t> Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and P ≤ 0.0001 (****). TCR = T cell receptor, PBMC = Peripheral Blood Mononuclear Cells, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher.
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Composition of αβ TCR+ T cell subpopulations in PBMC of naïve chickens. ( A ) Flow cytometry gating scheme for analysis of αβ TCR+ T cells. ( B ) Kinetics of αβ TCR+ T cell subtypes in PBMC of naïve adult chickens from the 6th until the 60th week of age. Upper left: cells expressing CD8α among all αβ TCR expressing cells, upper right: proportion of CD8α+ cells expressing CD8β, lower left: cells expressing CD4 among all αβ TCR expressing cells and lower right: proportion of CD4+ cells expressing <t>CD25.</t> Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and P ≤ 0.0001 (****). TCR = T cell receptor, PBMC = Peripheral Blood Mononuclear Cells, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher.
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Composition of αβ TCR+ T cell subpopulations in PBMC of naïve chickens. ( A ) Flow cytometry gating scheme for analysis of αβ TCR+ T cells. ( B ) Kinetics of αβ TCR+ T cell subtypes in PBMC of naïve adult chickens from the 6th until the 60th week of age. Upper left: cells expressing CD8α among all αβ TCR expressing cells, upper right: proportion of CD8α+ cells expressing CD8β, lower left: cells expressing CD4 among all αβ TCR expressing cells and lower right: proportion of CD4+ cells expressing <t>CD25.</t> Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and P ≤ 0.0001 (****). TCR = T cell receptor, PBMC = Peripheral Blood Mononuclear Cells, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher.
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Composition of αβ TCR+ T cell subpopulations in PBMC of naïve chickens. ( A ) Flow cytometry gating scheme for analysis of αβ TCR+ T cells. ( B ) Kinetics of αβ TCR+ T cell subtypes in PBMC of naïve adult chickens from the 6th until the 60th week of age. Upper left: cells expressing CD8α among all αβ TCR expressing cells, upper right: proportion of CD8α+ cells expressing CD8β, lower left: cells expressing CD4 among all αβ TCR expressing cells and lower right: proportion of CD4+ cells expressing CD25. Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and P ≤ 0.0001 (****). TCR = T cell receptor, PBMC = Peripheral Blood Mononuclear Cells, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher.

Journal: Poultry Science

Article Title: Baseline immune profiles of local chicken breeds: linking biodiversity, animal health, and vaccination response

doi: 10.1016/j.psj.2025.105565

Figure Lengend Snippet: Composition of αβ TCR+ T cell subpopulations in PBMC of naïve chickens. ( A ) Flow cytometry gating scheme for analysis of αβ TCR+ T cells. ( B ) Kinetics of αβ TCR+ T cell subtypes in PBMC of naïve adult chickens from the 6th until the 60th week of age. Upper left: cells expressing CD8α among all αβ TCR expressing cells, upper right: proportion of CD8α+ cells expressing CD8β, lower left: cells expressing CD4 among all αβ TCR expressing cells and lower right: proportion of CD4+ cells expressing CD25. Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and P ≤ 0.0001 (****). TCR = T cell receptor, PBMC = Peripheral Blood Mononuclear Cells, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher.

Article Snippet: Chicken CD25 , AV142 , Pure , Bio-Rad AbD serotec , 1:1000.

Techniques: Flow Cytometry, Expressing

Composition of T cell subpopulations in PBMC of naïve 35-week-old laying hens at peak performance, comparing local breeds and commercial lines. ( A ) γδ TCR+ T cell subtypes. Left figure: proportion of all γδ TCR expressing T cells among all CD3+ T cells and right figure: proportion of CD8+ γδ T cells. ( B ) αβ TCR+ T cell subtypes. Upper left: cells expressing CD8α among all αβ TCR expressing cells, upper right: proportion of CD8α+ cells expressing CD8β, lower left: cells expressing CD4 among all αβ TCR expressing cells and lower right: proportion of CD4+ cells expressing CD25. Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and P ≤ 0.0001 (****). PBMC = Peripheral Blood Mononuclear Cells, TCR = T cell receptor, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher, WR = White Rock, RG = Ranger.

Journal: Poultry Science

Article Title: Baseline immune profiles of local chicken breeds: linking biodiversity, animal health, and vaccination response

doi: 10.1016/j.psj.2025.105565

Figure Lengend Snippet: Composition of T cell subpopulations in PBMC of naïve 35-week-old laying hens at peak performance, comparing local breeds and commercial lines. ( A ) γδ TCR+ T cell subtypes. Left figure: proportion of all γδ TCR expressing T cells among all CD3+ T cells and right figure: proportion of CD8+ γδ T cells. ( B ) αβ TCR+ T cell subtypes. Upper left: cells expressing CD8α among all αβ TCR expressing cells, upper right: proportion of CD8α+ cells expressing CD8β, lower left: cells expressing CD4 among all αβ TCR expressing cells and lower right: proportion of CD4+ cells expressing CD25. Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and P ≤ 0.0001 (****). PBMC = Peripheral Blood Mononuclear Cells, TCR = T cell receptor, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher, WR = White Rock, RG = Ranger.

Article Snippet: Chicken CD25 , AV142 , Pure , Bio-Rad AbD serotec , 1:1000.

Techniques: Expressing